mouse icam-1 Search Results


93
Miltenyi Biotec anti mouse
Anti Mouse, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems 1 mouse icam 1
1 Mouse Icam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems recombinant mouse icam
Recombinant Mouse Icam, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems mouse icam 1 elisa
Mouse Icam 1 Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti mouse icam 1 cd54 antibody
Anti Mouse Icam 1 Cd54 Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems anti icam 1
Anti Icam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems elisa
Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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R&D Systems icam 1
Icam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio X Cell alexafluor647 anti human icam 1
Alexafluor647 Anti Human Icam 1, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Novus Biologicals recombinant mouse icam
Recombinant Mouse Icam, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Elabscience Biotechnology elisa mouse icam
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OriGene intracellular adhesion molecule 1 icam 1 phycoerythrin pe clone ha58
Figure 3. Toll-like receptor (TLR) agonists induce up-regulation of intercellular adhesion molecule 1 <t>(ICAM-1)</t> in Detroit-562. The cells were stimulated with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 6 or 24 hr. Thereaf- ter, the cells were stained with monoclonal antibodies against ICAM- 1 and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated. (a) ICAM-1 expression after stimulation for 24 hr. (b) Time-dependent up-regulation of ICAM-1 by poly(I:C) (C = control; n = 4; *P < 005; **P < 001; ***P < 0001).
Intracellular Adhesion Molecule 1 Icam 1 Phycoerythrin Pe Clone Ha58, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3. Toll-like receptor (TLR) agonists induce up-regulation of intercellular adhesion molecule 1 (ICAM-1) in Detroit-562. The cells were stimulated with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 6 or 24 hr. Thereaf- ter, the cells were stained with monoclonal antibodies against ICAM- 1 and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated. (a) ICAM-1 expression after stimulation for 24 hr. (b) Time-dependent up-regulation of ICAM-1 by poly(I:C) (C = control; n = 4; *P < 005; **P < 001; ***P < 0001).

Journal: Immunology

Article Title: Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.

doi: 10.1111/j.1365-2567.2008.03041.x

Figure Lengend Snippet: Figure 3. Toll-like receptor (TLR) agonists induce up-regulation of intercellular adhesion molecule 1 (ICAM-1) in Detroit-562. The cells were stimulated with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 6 or 24 hr. Thereaf- ter, the cells were stained with monoclonal antibodies against ICAM- 1 and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated. (a) ICAM-1 expression after stimulation for 24 hr. (b) Time-dependent up-regulation of ICAM-1 by poly(I:C) (C = control; n = 4; *P < 005; **P < 001; ***P < 0001).

Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry analysis: intracellular adhesion molecule 1 (ICAM-1)-phycoerythrin (PE) (clone HA58) and epithelial cell adhesion molecule (EpCAM)-PE (1B7) from eBioscience (San Diego, CA), unlabelled TLR1 (GD2.F4) from Acris Antibodies (Hiddenhausen, Germany), TLR2-PE (TL2.1), TLR3-PE (40C1285), TLR4-fluorescein isothiocyanate (FITC) (HTA125) and TLR5-PE (85B152.5) from AMS Biotechnology (Abingdon, UK) and ICAM-1-fluorescein (BBIG-I1) from R&D Systems.

Techniques: Staining, Bioprocessing, FACS, Expressing, Control

Figure 6. Responses induced by Toll-like receptor (TLR) ligands in human nasal epithelial cells (HNECs). (a) HNECs were analysed for expres- sion of TLR mRNA using real-time reverse transcription–polymerase chain reaction (RT-PCR). The values are expressed in relation to the house- keeping gene b-actin (n = 5). (b) The cells were cultured with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 24 hr. Thereafter, cells were stained with monoclonal antibodies against intercellular adhesion molecule 1 (ICAM-1) and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated (n = 4). (c, d, e) After stimulation, the cell culture supernatants were analysed for (c) interleukin (IL)-1b, (d) IL-6 and (e) IL-8 production by enzyme-linked immunosorbent assay (ELISA) (C = control; n = 6; *P < 005; **P < 001).

Journal: Immunology

Article Title: Toll-like receptor agonists induce inflammation and cell death in a model of head and neck squamous cell carcinomas.

doi: 10.1111/j.1365-2567.2008.03041.x

Figure Lengend Snippet: Figure 6. Responses induced by Toll-like receptor (TLR) ligands in human nasal epithelial cells (HNECs). (a) HNECs were analysed for expres- sion of TLR mRNA using real-time reverse transcription–polymerase chain reaction (RT-PCR). The values are expressed in relation to the house- keeping gene b-actin (n = 5). (b) The cells were cultured with or without Pam3CSK4, poly(I:C), flagellin or tumour necrosis factor (TNF)-a (10 ng/ml) for 24 hr. Thereafter, cells were stained with monoclonal antibodies against intercellular adhesion molecule 1 (ICAM-1) and analysed by fluorescence-activated cell sorting (FACS). The mean fluorescence intensity (MFI) of the ICAM-1-positive cells was calculated (n = 4). (c, d, e) After stimulation, the cell culture supernatants were analysed for (c) interleukin (IL)-1b, (d) IL-6 and (e) IL-8 production by enzyme-linked immunosorbent assay (ELISA) (C = control; n = 6; *P < 005; **P < 001).

Article Snippet: The following monoclonal antibodies (mAbs) were used for flow cytometry analysis: intracellular adhesion molecule 1 (ICAM-1)-phycoerythrin (PE) (clone HA58) and epithelial cell adhesion molecule (EpCAM)-PE (1B7) from eBioscience (San Diego, CA), unlabelled TLR1 (GD2.F4) from Acris Antibodies (Hiddenhausen, Germany), TLR2-PE (TL2.1), TLR3-PE (40C1285), TLR4-fluorescein isothiocyanate (FITC) (HTA125) and TLR5-PE (85B152.5) from AMS Biotechnology (Abingdon, UK) and ICAM-1-fluorescein (BBIG-I1) from R&D Systems.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Cell Culture, Staining, Bioprocessing, FACS, Enzyme-linked Immunosorbent Assay, Control